software carestream mi se  (Informer Technologies Inc)

Journal: PLoS ONE

Article Title: mTOR Inhibition by Everolimus in Childhood Acute Lymphoblastic Leukemia Induces Caspase-Independent Cell Death

doi: 10.1371/journal.pone.0102494

Figure Lengend Snippet: ( A ) NALM6 cells were treated with 16 µM everolimus overnight and qRT-PCR used to evaluate the expression of the indicated genes. The mean ± SD of the fold change from 3 experiments is shown. ( B ) NALM6 cells were incubated with the indicated concentrations of everolimus for the specified time periods and cell lysates prepared. Sequential Western blotting was performed on the same membrane to determine the levels of the indicated proteins in each series of panels. Phospho-eIF2α bands were quantitated by densitometry and normalized to β-actin.

Article Snippet: Bands were quantitated by densitometry (Molecular Dynamics) using CareStream MI SE software.

Techniques: Quantitative RT-PCR, Expressing, Incubation, Western Blot, Membrane

Journal: PLoS ONE

Article Title: mTOR Inhibition by Everolimus in Childhood Acute Lymphoblastic Leukemia Induces Caspase-Independent Cell Death

doi: 10.1371/journal.pone.0102494

Figure Lengend Snippet: ( A ) NALM6 cells were treated for the indicated time with 16 µM everolimus and cell lysates prepared. Lysates were probed for phosphorylated JNK (p-JNK) and loading assessed using β-actin. ( B ) NALM6 cells were treated with the indicated concentrations of everolimus for 16 h, with or without a 1 h pre-incubation with the JNK inhibitor SP600125 (5 µM) and viability assessed using annexin V/PI staining. The percentage of viable cells is shown with the bars representing the mean ± SE of 2 experiments. ( C ) NALM6 or REH cells were incubated with indicated concentrations of cyclohexamide for 1 h prior to the addition of the indicated concentration of everolimus. The mean ± SD of ≥3 independent experiments is shown. *p<0.05 when comparing cells with and without cyclohexamide. ( D ) NALM6 cells were treated with the indicated concentrations of everolimus for the specified times and cell lysates prepared. The indicated proteins were assessed by Western blotting and where indicated the bands were quantitated by densitometry using β-actin for normalization.

Article Snippet: Bands were quantitated by densitometry (Molecular Dynamics) using CareStream MI SE software.

Techniques: Incubation, Staining, Concentration Assay, Western Blot